Reproductive Performance of Mouse Oocyte after In Vivo Exposure of The Ovary to Continuous Wave Ultrasound
نویسندگان
چکیده
BACKGROUND There is a lack of studies regarding the effects of ultrasound (US) and replication of its exposure on pre-implantation events in mammals. Thus, this study assesses the reproductive performance of mouse oocytes that have been obtained from ovaries irradiated with US waves versus non-irradiated ovaries. Also comparision of their parthenogenesis, ovulation, fertilization, and pre-implantation development rates. MATERIALS AND METHODS In this experimental study, we divided extracted ovaries into three experimental groups that received the same dosage, but different replicates of radiation for each group. Results were compared with the control and sham groups. Continuous wave (CW) US, at a spatial average intensity of 355 mW/cm(2) and a frequency of 3.28 MHz, was administered for 5 minutes to the ovaries at an interval between pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) injections. Statistical analysis was performed using the ANOVA test and the level of significance was determined to be 0.05. RESULTS Data collection was based on microscopic visualization. According to the obtained results, metaphase II (MII) oocyte numbers and the percentage of blastocysts significantly reduced in the USexposed groups versus the unexposed groups. Fertilization rate was comparable between groups while parthenogenesis was significantly higher in the US-exposed groups compared to the unexposed groups. CONCLUSION Structural damage to cells, intracellular organelles and proteins, as well as changes in signaling pathways induced by US may be reasons for some of the observed adverse effects in groups that have received more US exposure.
منابع مشابه
Reproductive Performance of Mouse Oocyte after In Vivo Exposure of The Ovary to Continuous Wave Ultrasound
متن کامل
P-217: The Effect of Progesterone on The In Vitro Maturation of Mouse Germinal Vesicle Oocytes Derived from Polycystic Ovary
Background: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation and hyperandro genism and affects approximately 5-10% of women of reproductive age. In vitro maturation (IVM) of mammalian oocytes has become an efficient method to produce mature oocytes in order to use in assisted reproductive techniques such as in vitro fertilization (IVF), intra cytoplasmic sperm injection ...
متن کاملP-254: The Effects of Zinc in Vitrification Medium on In vitro Maturation of Oocytes Derived from Vitrified-Warmed Mouse Ovary
Background: Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. Researchers have used different cryoprotectants and various techniques to improve the cryopreservation of ovaries However, despite significant recent progress, the efficiency of ovary cryopreservation is still low. Zinc is essential for ...
متن کاملP-255: The Effects of Vanadium in Vitrification Medium on in vitro Maturation of Oocytes Derived from Vitrified-Warmed Mouse Ovary
Background: Since the freezing method was offered, much effort has been done to improve the freezing conditions in different studies and the impact of different freezing methods and mediums on oocyte, embryo and ovarian tissues has been examined. Based on studies, minerals play an important role in oocyte maturation and subsequent embryo development. Vanadium is one of the minerals, which is ve...
متن کاملP-198: Study Effects of EMF on Pathology of Ovary in Newborn Rats during Developmental Period
Background: The effect of electromagnetic field (EMF) as an environmental factor on different organs including female reproductive system is of critical concern. In view of the fact that embryo fetuses and young, growing animals are more susceptible to any xenobiotic during gestational and lactation stages of gonadal development may it lead to permanent damage to the gonads. The early phases of...
متن کامل